By Adele Madden


Clodronate is very effective in the treatment of hypercalcemia and cancer metastases to bones. It can significantly reduce the development of new metastases, as well as to decrease the risk of bone fractures, which is very high in such cases. Besides, it may also reduce the pain associated with this disease. Clodronate liposome, also known as clodrosome, seems to be the best way of delivering this compound to tissues.

Liposomes are lipid-based nanoparticles. Liposomal encapsulated drugs are mostly used in academic research laboratories. Some new anti-cancer compounds such as this one require a liposome delivery system to be closed in vivo. Target organs for this particular compound are kidneys and bones.

Clodronate liposome suspension should be injected to targeted tissues. While manipulating this type of suspension, some measures of precaution are necessary, such as chemical resistant gloves and clothing, safety goggles and respirators. This compound can be harmful if inhaled, absorbed through skin, swallowed or if it get in contact with your eyes. It causes different irritations, especially to mucous membranes, and you should be very careful with it.

If something like this happens, you need to follow these procedures. If it comes in contact with your eyes, you should wash them for at least fifteen minutes. Skin should be cleaned with soap and plenty of water. You should have respirator to use it if you inhale it, and you should go outside right away. If the person who inhaled it have more serious breathing problems, you should give him an oxygen. If you happen to swallow it, you should call a physician immediately.

Macrophages are very important, because they regulate immune reactions and some other, non-phagocytic cells function. Their primary function is to ingest and digest unfamiliar macromolecules and other microorganisms. In order to override their function, they use liposomal encapsulated compounds and drugs.

When clodronate is encapsulated within liposomes, it can be delivered to bones and kidneys. Macrophages will digest those particles, but this compound will ve released and accumulated in tissues. Creating macrophage depleted tissues and organs this way allows more successful research of macrophage functional aspects.

Clodronate liposomes application can also be used for suppression of macrophage activity in different models of autoimmune diseases, in gene therapy and similar researches. Although this method is designed especially for in vivo research, it can also be successfully used for depletion of macrophages in vitro.

The suspension should be kept at 4 degrees of Celsius, up to one month. The important thing to remember is that it should never be frozen, or exposed to temperatures over 30 degrees. It is also important to wait until this suspension reach room temperature before injecting it to your animals. Other ways your results could be different than expected.

It is also important to shake the suspension until it becomes homogeneous. You should shave the skin and cleanse it with alcohol, to make sure it is disinfected. You should use sterile syringe for each animal. If you use the same one, you might get uneven dosages. Follow the procedure for injecting clodronate liposome to your test animals.




About the Author:



0 commentaires:

Post a Comment

Related Posts Plugin for WordPress, Blogger...